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溝口 明

当研究室では昆虫の成長、変態、休眠を調節するホルモンの研究も行っています。昆虫の脱皮・変態は前胸腺と呼ばれる内分泌腺が分泌するエ クジステロイド （脱皮ホルモン）により支配されていますが、同ホルモンの分泌の時期と量は中枢神経系が分泌する様々なペプチドホルモンにより調節されて います。そしてこ の調節により、昆虫は一定の大きさに成長して初めて脱皮や変態をすることができ、また、生存に不適な季節を生き延びるために発育を停止 （休眠）できるよう になっています。一方、昆虫の成長の調節にはインスリンやインスリン様成長因子によく似たホルモンが重要な働きをします。私たちはこれら のペプチドホルモ ンの研究を通して、成長と調和した変態誘導のしくみや環境適応的な発育調節のしくみを明らかにしたいと考えています。

参考文献

  1. Okamoto N, Yamanaka N, Endo Y, Kataoka H, Mizoguchi A.: Spatiotemporal patterns of IGF-like peptide expression in the silkmoth Bombyx mori predict its pleiotropic actions. Gen Comp Endocrinol. 2011 Aug 1;173(1):171-82.

  2. Yamanaka N, Roller L, Zitňan D, Satake H, Mizoguchi A, Kataoka H, Tanaka Y: Bombyx orcokinins are brain-gut peptides involved in the neuronal regulation of ecdysteroidogenesis. J Comp Neurol. 2011 Feb 1;519(2):238-46.

  3. Yamanaka, N., Hua, Y. -J., Roller, L., Spalovská-Valachová, I., Mizoguchi, A., Kataoka, H., Tanaka, Y. (2010) Bombyx prothoracicostatic peptides activate the sex peptide receptor to regulate ecdysteroid biosynthesis. Proc. Natl. Acad. Sci. USA 107: 2060-2065.

  4. Okamoto, N., Yamanaka, N., Yagi, Y., Nishida, Y., Kataoka, H., O'Connor, M.B., Mizoguchi, A.: A fat body-derived IGF-like peptide regulates postfeeding growth in Drosophila. Dev. Cell (2009) 6 885-891.

  5. Okamoto, N., Yamanaka, N., Satake, H., Saegusa, H., Kataoka, H., Mizoguchi, A.: A fat body-derived An ecdysteroid-inducible insulin-like growth factor-like peptide regulates adult development of the silkmoth Bombyx mori. FEBS J (2009) 276 1221-1232.

  6. Yamanaka, N., Zitnan, D., Kim, Y. –J., Hua, Y. -J., Suzuki, Y., Suzuki, M., Suzuki, A., Satake, H., Mizoguchi, A., Asaoka, K., Tanaka, Y. and Kataoka, H. (2006) Regulation of insect steroid hormone biosynthesis by innervating peptidergic neurons. Proc. Natl. Acad. Sci. USA 103: 8622-9627.

  7. Kim, Y. –J., Zitnan, D., Cho, K. –H., Schooley, D. A., Mizoguchi, A., Adams, M. E. (2006) Central peptidergic ensembles associated with organization of an innate behavior. Proc. Natl. Acad. Sci. USA, 103: 14211-14216

  8. Mizoguchi A, Kataoka H.: An in vitro study on regulation of prothoracic gland activity in the early last-larval instar of the silkworm Bombyx mori. J Insect Physiol. 2005 51(8):871-9.

  9. Mizoguchi A, Dedos SG, Fugo H, Kataoka H.: Basic pattern of fluctuation in hemolymph PTTH titers during larval-pupal and pupal-adult development of the silkworm, Bombyx mori. Gen Comp Endocrinol. 2002 127(2):181-9.

  10. Mizoguchi A.: Effects of juvenile hormone on the secretion of prothoracicotropic hormone in the last- and penultimate-instar larvae of the silkworm Bombyx mori. J Insect Physiol. 2001 47(7):767-775.

  11. Mizoguchi A, Ohashi Y, Hosoda K, Ishibashi J, Kataoka H.: Developmental profile of the changes in the prothoracicotropic hormone titer in hemolymph of the silkworm Bombyx mori: correlation with ecdysteroid secretion. Insect Biochem Mol Biol. 31(4-5):349-58 (2001).

  12. Satake S, Kawabe Y, Mizoguchi A.: Carbohydrate metabolism during starvation in the silkworm Bombyx mori. Arch Insect Biochem Physiol. 44(2):90 (2000)

  13. Gilbert, L. I., Rybczynski, R., Song, Q., Mizoguchi, A., Morreale R., Smith, W. A., Matubayashi, H., Shionoya, M., Nagata, S. and Kataoka, H.: Dynamic regulation of prothoracic gland ecdysteroidogenesis: Manduca sexta recombinant prothoracicotropic hormone and brain extracts have identical effects. Insect Biochem. Mol. Biol. 30: 1079-1089 (2000).

  14. Masumura, M., Satake, S., Saegusa, H. and Mizoguchi, A.: Glucose stimulates the release of bombyxin, an insulin-related peptide of the silkworm, Bombyx mori. General Comp. Endocrinol. 118: 393-399 (2000).

  15. Satake S., Nagata K., Kataoka H. and Mizoguchi A.: Bombyxin secretion in the adult silkmoth Bombyx mori: sex-specificity and its correlation with metabolism. J. Insect Physiol. 45: 939-945 (1999).