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CRYOPRESERVATION

Strussmann CA, Nakatsugawa H, Takashima F, Hasobe M, Suzuki T, Takai R
Cryopreservation of Isolated Fish Blastomeres: Effects of Cell Stage, Cryoprotectant Concentration, and Cooling Rate on Postthawing Survival.
Cryobiology. 1999 Nov;39(3):252-261.
Krone A. and Wittbrodt, J. 1997
A simple and reliable protocol for cryopreservation of Medaka (Oryzias latipes) spermatozoa
Fish Biol. J. Medaka 9 : 47-45 (1997).

Aoki K, Okamoto M, Tatsumi K and Ishikawa Y, 1997
Cryopreservation of medaka spermatozoa.
Zool Sci 14 (4), 641-644 (1997)