研究内容


 

研究業績


Recent Publications (2000-2010)

Akasaka, M., Harada, Y., and Sawada, H. (2010). Vitellogenin C-terminal fragments participate in fertilization as egg-coat binding partners of sperm trypsin-like proteases in the ascidian Halocynthia roretzi.
Biochem. Biophys. Res. Commun. 392 (4), 479-484. pubmed

Yamada, L., Saito, T., Taniguchi, H., Sawada, H., and Harada, Y. (2009). Comprehensive egg coat proteome of the ascidian Ciona intestinalis reveals gamete recognition molecules involved in self-sterility.
J. Biol. Chem. 284 (14), 9402-9410. pubmed

原田淑人 (2009). ホヤで自己・非自己識別機構を探る
研究をささえるモデル生物（吉川寛・堀寛 著、化学同人） 101-103.（総説）

原田淑人，澤田　均 (2008). 雌雄同体の脊索動物であるホヤの自家不稔性のメカニズム
細胞工学 27 (9), 906-907.（総説）

Harada, Y., and Sawada, H. (2008). Allorecognition mechanisms during ascidian fertilization.
Int. J. Dev. Biol. 52 (5-6), 637-645. (review) pubmed

Urayama, S., Harada, Y., Nakagawa, Y., Ban, S., Akasaka, M., Kawasaki, N., and Sawada, H. (2008). Ascidian sperm glycosylphosphatidylinositol-anchored CRISP-like protein as a binding partner for an allorecognizable sperm receptor on the vitelline coat.
J. Biol. Chem. 283 (31), 21725-21733. pubmed

Hirohashi, N., Kamei, N., Kubo, H., Sawada, H., Matsumoto, M., and Hoshi, M. (2008). Egg and sperm recognition systems during ferilization.
Dev. Growth Differ. 50 (Suppl 1), S221-S238 (review). pubmed

Harada, Y., Takagaki, Y., Sunagawa, M., Saito, T., Yamada, L., Taniguchi, H., and Shoguchi, E., and Sawada, H. (2008). Mechanism of self-sterility in a hermaphroditic chordate.
Science 320 (5875), 548-550. pubmed

Kikushima, E., Nakamura, S., Oshima, Y., Shibuyaa, T., Miao, J.Y., Hayashi, H., Nikai, T., and Araki, S. (2008). Hermorrhagic activity of the vascular apoptosis-inducing proteins VAP1 and VAP2 from Crotalus atrox.
Toxicon 52 (4), 589-593. pubmed

Harada, Y., and Sawada, H. (2007). Proteins interacting with the ascidian vitelline-coat sperm receptor HrVC70 as revealed by yeast two-hybrid screening.
Mol. Reprod. Dev. 74 (9), 1478-1487. pubmed

Yokota, N., and Sawada, H. (2007). Sperm proteasomes are responsible for the acrosome reaction and sperm penetration of the vitelline envelope during fertilization of the sea urchin Pseudocentrotus depressus.
Dev. Biol. 308 (1), 222-231. pubmed

Yokota, N., and Sawada, H. (2007). Effects of proteasome inhibitors on fertilization of the sea urchin Anthocidaris crassispina.
Biol. Pharm. Bull. 30 (7), 1332-1335. pubmed

Masuda, S., Maeda, H., Miao, J.Y., Hayashi, H., and Araki, S. (2007). cDNA cloning and some additional peptide characterization of a single-chain vascular apoptosis-inducing protein, VAP2.
Endothelium 14 (2), 89-96. pubmed

Igarashi, T., Araki, S., Mori, H., and Takeda, S. (2007). Crystal structures of catrocollastatin/ VAP2B reveal a dynamic, modular architecture of ADAM/adamalysin/reprolysin family proteins.
FEBS Lett. 581 (13), 2416-2422. pubmed

Takagi Sawada, M., Tamura T., Mitani, Y., Kaya, M., Ito, G., Hashimoto, H., and Sawada, H. (2006). Participation of proteasome-associating complex PC500 in starfish oocyte maturation as revealed by monoclonal antibodies.
Biochem. Biophys. Res. Commun. 349 (2), 694-700. pubmed

澤田　均 (2006). 受精におけるプロテアソーム
「精子学（毛利秀雄・星元紀監修） pp.147-152., 東京大学出版会（総説）

Cheng, Y., Zhao, Q., Liu, X., Araki, S., Zhang, S., and Miao, J. (2006). Phosphatidylcholine-specific phospholipase C, p53 and ROS in the association of apoptosis and senescence in vascular endothelial cells.
FEBS Lett. 580 (20), 4911-4915. pubmed

Liu, X., Zhao, Q., Araki, S., Zhang, S., Miao, J. (2006). Contrasting effects of phosphatidylinositol- and phosphatidylcholine-specific phospholipase C on apoptosis in cultured endothelial cells.
Endothelium 13 (3), 205-211. pubmed

Igarashi, T., Oishi, Y., Araki, S., Mori, H., Takeda, S. (2006). Crystallization and preliminary X-ray crystallographic analysis of two vascular apoptosis-inducing proteins (VAPs) from Crotalus atrox venom.
Acta Crystallogr. Sect. F. Struct. BIol. Cryst. Commun. 62 (Pt 7), 688-691. pubmed

Takeda, S., Igarashi, T., Mori, H., and Araki, S. (2006). Crystal structures of VAP1 reveal ADAMs' MDC domain architecture and its unique C-shaped scaffold.
EMBO J. 25 (11), 2388-2396. pubmed

Ban, S., Harada, Y., Yokosawa, H., and Sawada, H. (2005). Highly polymorphic vitelline-coat protein HaVC80 from the ascidian, Halocynthia aurantium: Structural analysis and involvement in self/nonself recognition during fertilization.
Dev. Biol. 286 (2), 440-451. pubmed

Sawada, H., Akasaka, M., Yokota, N., and Sakai, N.(2005). Modifications of ascidian fertilization related gamete proteins by ubiquitination, proteolysis, and glycosylation.
In “New Impact on Protein Modifications in the Regulation of Reproductive System. (T. Tokumoto ed.)”pp. 61-81, Research Signpost, Kerala, India . (review)

Maruyama, J., Hayashi, H., Miao, J., Sawada, H., and Araki, S.(2005). Severe cell fragmentation in the endothelial cell apoptosis induced by snake toxin VAP1 is an apoptotic characteristic controlled by caspase.
Toxicon 46 (1), 1-6. pubmed

Sawada, H., Tanaka, E., Ban, S., Yamasaki, C., Fujino, J., Ooura, K., Abe, Y., Matsumoto, K., and Yokosawa, H. (2004). Self/nonself recognition in ascidian fertilization: vitelline coat protein HrVC70 is a candidate allorecognition molecule.
Proc. Natl. Acad. Sci. USA, 101 (44), 15615-15620. pubmed

Sawada, H., Kikukawa, Y., Ban, S., Kakudo, T., and Yokosawa, H. (2004). Expression of trypsin-like proteases and protease nexin-1 in mdx mouse muscles.
Biochem. Biophys. Res. Commun. 314 (2), 654-658. pubmed

Sakai, N., Takagi Sawada, M., and Sawada, H. (2004). Non-traditional roles of ubiquitin-proteasome system in fertilization and gametogenesis.
Int. J. Biochem. Cell Biol. 36 (5), 776-84. pubmed

Sawada , H. (2004). Spermosin.
In Handbook of Proteolytic Enzymes (A.J. Barrett, N.D. Rawlings, J.F. Woessner eds.) pp. 1567-1569, Elsevier-Academic Press, Amsterdam.

Zhao, Q., Araki, S., Zhang, S., and Miao, J. (2004). Rattlesnake venom induces apoptosis by stimulating PC-PLC and upregulating the expression of integrin beta4, P53 in vascular endothelial cells.
Toxicon 44 (2), 161-168. pubmed

Sakai, N., Sawada, H., and Yokosawa, H. (2003). Extracellular ubiquitin system implicated in fertilization of the ascidian, Halocynthia roretzi: isolation and characterization.
Dev Biol. 264 (1), 299-307. pubmed

Sawada, H., Nagahiro, K., Kikukawa, Y., Ban, S., Kakefuda, R., Shiomi, T., and Yokosawa, H. (2003). Therapeutic effect of camostat mesilate on Duchenne muscular dystrophy in mdx mice.
Biol. Pharm. Bull. 26, 1025-1027. pubmed

Du, C., Zhao, Q., Araki, S., Zhang, S., and Miao, J. (2003). Apoptosis mediated by phosphatidylcholine-specific phospholipase C is associated with cAMP, p53 level, and cell-cycle distribution in vascular endothelial cells.
Endothelium 10 (3), 141-147. pubmed

Sawada, H., Sakai, N., Abe, Y., Tanaka, E., Takahashi, Y., Fujino, J., Kodama, E., Takizawa, S., and Yokosawa, H. (2002). Extracellular ubiquitination and proteasome-mediated degradation of the ascidian sperm receptor.
Proc. Natl. Acad. Sci. USA 99 (3), 1223-1228. pubmed

Kodama, E., Baba, T., Kohno, N., Satoh, S., Yokosawa, H., and Sawada, H. (2002). Spermosin, a trypsin-like protease from ascidian sperm: cDNA cloning, protein structures and functional analysis.
Eur. J. Biochem. 269 (2), 657-663. pubmed

Sawada, H., Takahashi, Y., Fujino, J., Flores, S. Y., and Yokosawa, H. (2002). Localization and roles in fertilization of sperm proteasomes in the ascidian Halocynthia roretzi.
Mol. Reprod. Dev. 62 (2), 271-276. pubmed

Sawada, H. (2002). Ascidian sperm lysin system.
Zool. Sci. 19 (2), 139-151. (review) pubmed

Lambert, C.C., Someno, T., and Sawada H. (2002). Sperm surface proteases in ascidian fertilization.
J. Exp. Zool. 292 (1), 88-95. pubmed

Araki, S., Masuda, S., Maeda, H., Ying, M.J., and Hayashi, H. (2002). Involvement of specific integrins in apoptosis induced by vascular apoptosis-inducing protein 1.
Toxicon 40 (5), 535-542. pubmed

Satoh, K., Sasajima, H., Nyoumura, K., Yokosawa, H., and Sawada, H. (2001). Assembly of the 26S proteasome is regulated by phosphorylation of the p45/Rpt6 ATPase subunit.
Biochemistry 40, 314-319. pubmed

Kodama, E., Baba, T., Yokosawa, H., and Sawada, H. (2001). cDNA cloning and functional analysis of ascidian sperm proacrosin.
J. Biol. Chem. 276 (27), 24594-24600. pubmed

Sawada, H., and Yokosawa, H. (2001). Self-nonself recognition and lysin-system in fertilization of the ascidian Halocynthia roretzi.
In The Biology of Ascidians (H. Sawada, H. Yokosawa, and C. C. Lambert, eds.), pp. 18-23, Springer-Verlag, Tokyo.

Kodama, E., Baba, T., Yokosawa, H., and Sawada, H. (2001). Ascidian sperm acrosin and spermosin: Structures and possible roles in fertilization.
In The Biology of Ascidians (H. Sawada, H. Yokosawa, and C. C. Lambert, eds.), pp. 54-59, Springer-Verlag, Tokyo.

沢田　均 (2001). プロテアソームと制御因子の結晶構造．PA28は本当にプロテアソームアクチベーターか？
蛋白質 核酸 酵素 46, 264-265

沢田　均 (2001). 卵成熟と受精．
医学のあゆみ 198, 63-68（プロテアーゼとそのインヒビター−最新の知見）

Masuda, S., Hayashi, H., Atoda, H., Morita, T., and Araki S. (2001). Purification, cDNA cloning and characterization of the vascular apoptosis-inducing protein, HV1, from Trimeresurus flavoviridis.
Eur. J. Biochem. 268 (11), 3339-3345. pubmed

Tanaka, E., Takagi Sawada, M., Morinaga, C., Yokosawa, H., and Sawada, H. (2000). Isolation and characterization of a novel 530-kDa protein complex (PC530) capable of associating with the 20S proteasome from starfish oocytes.
Arch. Biochem. Biophys. 374, 181-188. pubmed

Tanaka, E., Takagi Sawada, M., and Sawada, H. (2000). Enzymatic properties of the proteasome purified from starfish oocytes and its catalytic subunits involved in oocyte maturation.
Comp. Biochem. Physiol. Part C 125, 215-223. pubmed

Morinaga, C., Izumi, K., Sawada, H., and Takagi Sawada, M. (2000). Activation of maturation promoting factor and 26S proteasome assembly accelerated by a high concentration of 1-methyladenine in starfish oocytes.
Biosci. Biotechnol. Biochem. 64, 268-274. pubmed

Sawada, H., Tanaka, E., Abe, Y., Takizawa, S., Takahashi, Y., Fujino, J., and Yokosawa, H. (2000). Ascidian sperm receptor attached to the vitelline coat during oocyte maturation.
Zygote 8, S14-15. pubmed

Miao, J.Y., Araki, S., Zhang, S.L., and Hayashi, H. (2000). 1-Methyl-3-isobutylxanthine delays apoptosis induced by deprivation of growth factors in vascular endothelial cells.
Acta Pharmacol. Sin. 21 (10), 936-938. pubmed