Home ＞GCOE Researchers ＞Takeshi MIZUNO

GCOE Researchers

Takeshi MIZUNO

Affiliation
/Position		 Graduate School of Bioagricultural Sciences, Department of Biological Mechanisms and Functions, Professor
Doctorate Doctor of Agriculture
Research interests interests: Molecular cell genetics, Molecular mechanisms of signal transduction and clock systems in higher plants
address tmizuno＠agr.nagoya-u.ac.jp
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+81-52-789-4089
  Laboratory

Outline of research

Our laboratory’s focus is the universality and diversity of molecular mechanisms of signal transduction system mediated by two-component signal transduction systems, which exist in a wide array of model organisms ranging from E. coli to higher plants. Our studies employ molecular biological techniques and have systematized knowledge of this field. We are currently expanding our analysis to the clock system in higher plants.

  1. Discovery of the two-component phosphorelay signal transduction system in bacteria: We discovered that a two-component phosphorelay signal transduction system, distinct from that of eukaryotes, exists in the intracellular signal transduction system of E. coli. We also demonstrated that this mechanism exists universally in a wide range of bacteria. In a related study, we discovered the first antisense RNA mechanism, marking the beginning of subsequent progression of RNAi researches.
  2. Universalization of the two-component phosphorelay signal transduction system in eukaryotes and the discovery of phytohormone cytokinin receptors: We demonstrated that the two-component phosphorelay signal transduction system also exists in eukaryotes (yeast and plant), and described its molecular mechanism in fission yeast. As part of the study, we identified a receptor for cytokinin, a plant growth hormone, and elucidated the molecular mechanisms of the hormone response.
  3. Discovery novel circadian clock genes in higher plants: Understanding the mechanism of circadian clock, which intricately controls many sophisticated functions in higher plants, has long been an important problem in plant biology. We discovered so-called "clock genes," which constitute the central oscillator of the clock, and revealed their functions. These clock genes (named PRRs) also play important roles in universal functions in higher plants, such as control of flowering time and adaptation to light. From the results of our studies, we proposed a new molecular model for the plant circadian clock.

References

  1. Ishida K. et al. (2008) Three hype-B response regulators, ARR1, ARR10, and ARR12 play essential but redundant roles in cytokinin signal transduction through the life cycle of Arabidopsis thaliana. Plant Cell Physiol., 49: 47-57.
  2. Ito S. et al. (2008) Insight into missing genetic links between tow evening-expressed pseudo-response regulator genes TOC1 and PRR5 in the circadian clock-controlled circuitry in Arabidopsis thaliana. Plant Cell Physiol., 49: 201-213.
  3. Mizuno T., & Yamshino T. (2008) Comparative ranscription of diurnally oscillating genes and hormone-responsive genes in Arabidopsis thaliana; Insight into circadian-clock daily responses to common ambient stresses in plants. Plant Cell Physiol., 49: 481-487.
  4. Yokoyama A. et al. (2007) Type-B ARR transritprion factors, ARR10 and ARR12, are implicated in cytokinin-mediated regulation of protoxylem differentiation in roots of Arabidopsis thaliana. Plant Cell Physiol., 48: 84-96.
  5. Murakami M. et al. (2007) Comparative overviews as to clock-associated genes of Arabidopsis thaliana and Oryza sativa. Plant Cell Physiol., 48: 110-121.
  6. Iwama A. et al. (2007) AHK5 His-kinase regulates root elongation through an ETR1-dependent integrated abscisic acid and ethylene signaling pathway in Arabidopsis thaliana. Plant Cell Physiol., 48: 375-380.
  7. Nakamichi N. et al. (2007) Arabidopsis clock-associated pseudo-response regulatorsPRR9, PRR7, and PRR5 coodinately and positively regulate flowering time through the canonical CONSTANS-dependent photoperiodic pathway. Plant Cell Physiol., 48: 822-832.
  8. It, S. et al. (2007) Genetic linkages between circadian clock-associated components and phytochrome-dependent red light-signal transduction in Arabidopsis thaliana. Plant Cell Physiol., 48: 971-983.
  9. Niwa Y. et al. (2007) Genetic linkages of the circadian clock-associated genes, TOC1, CCA1, and LHY, in the photoperiodic control of flowering time in Arabidopsis thaliana. Plant Cell Physiol., 48: 925-937.
  10. Ito S. et al. (2007) Rhythmic and light-inducible appearance of clock-associated pseudo-response regulator protein PRR9 through programmed degradation in the dark in Arabidopsis thaliana. Plant Cell Physiol., 48: 1644-1651.

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